Project ID No:
2025-1.3.3-NEURO-2025-00001 

Project title: 
Development of behavior-based neuromodulation technologies for the targeted modulation of sensory systems and neural networks.

Short summary of the project:

The aim of the project is to develop innovative molecular, genetic, and neurophotonics tools that contribute to the partial restoration of visual function and to a deeper understanding of cortical plasticity. One of the central challenges in neuroscience is how to restore sensory function in cases where the optic nerve is damaged or absent. Researchers at the BrainVisionCenter address this problem through a state-of-the-art, multidisciplinary approach that integrates chemistry, biology, engineering, and neuroscience.

A major development direction focuses on the synthesis of photolabile NGF-mimetic compounds that enable light-controlled modulation of cell growth and network connectivity. Such photoactivatable molecules may allow precise regulation of regenerative processes and support partial restoration of visual function. In parallel, we are developing a novel three-dimensional, real-time motion-corrected microscopy system capable of high-precision imaging in moving samples. Complementing this approach, low-toxicity DNA-binding dyes are being designed to enable long-term imaging of living cells.

The project also includes the further development of the Moculus virtual reality–based experimental platform, which tightly couples visual stimuli with behavioral responses, thereby enabling controlled investigation of vision-driven learning under laboratory conditions. Concurrently, we are establishing a real-time cortical pattern reactivation method designed to enhance visual perception and learning efficiency through the replay of neuronal activity patterns.

Our genetic development efforts involve the generation of novel recombinant adeno-associated viral (rAAV) vectors for the targeted delivery of next-generation optical sensors and light-sensitive proteins into defined neuronal populations. In addition, we are refining maintenance protocols for human brain tissue to facilitate the translational application of laboratory findings to human systems. A core basic research pillar of the project examines dendritic activity in cortical interneurons, providing new insight into how these cell types contribute to vision-driven learning and cortical plasticity. This knowledge is essential for establishing long-term, neuron-based strategies for visual rehabilitation.

A key strength of the project lies in the integration of chemical, genetic, technological, and fundamental research components, which operate synergistically rather than independently. The resulting comprehensive knowledge base may open new avenues for treating certain forms of blindness and for advancing research into other neurological disorders. The program will yield at least one documented and accessible laboratory innovation, peer-reviewed scientific publications, and new collaborative networks. Beyond its impact on the scientific community, the outcomes are expected to contribute in the longer term to socially relevant healthcare and technological innovations.

Project implementation period: 2025.11.01 – 2026.04.30

Project budget: 500.000.000 Ft

Project aid intensity: 100%

Project location:
1094 Budapest, Liliom u. 43.
1094 Budapest, Tűzoltó u. 59.
7673 Kővágószőlős 524/1.

 

Project ID No: 2025-3.1.1-ED-2025-00027

Project title: 
Development of a multimodal neuroscience platform for a mouse model: targeted neuronal labeling, cell type–specific learning mechanisms, realistic MoculusVR environment, and freely moving two-photon imaging integrated into complex behavioral paradigms.

Short summary of the project:

The aim of the project is to develop new tools that will help us understand in greater detail how the brain works during learning, perception, and behavior. Part of this involves further developing a special virtual reality (VR) system called Moculus, which we designed specifically for mice. This system allows animals to move in a true three-dimensional (3D) environment, which is essential for them to learn and navigate naturally. The goal is for this system to more accurately mimic the natural vision of mice, while becoming an easy-to-use, multi-instance device that can be used by international research groups.

In parallel, another line of research is investigating the neural basis of learning. We are paying particular attention to a special type of cell, VIP interneurons, which play a role in how sensory information is transformed in the brain when, for example, the rules of reward change. For such studies, we perform targeted genetic modification using special viral vectors (AAVs), which allow us to make individual nerve cells visible or controllable by light.

The third pillar of the project is the development of a new robotic arm optical system that allows us to perform two-photon imaging on mice even when they are moving freely. This is a breakthrough because it allows us to observe brain function under much more natural conditions. In addition to developing the system, we also aim to launch a domestic scientific collaboration to help introduce the new technology and ensure the physiological validity of the measurements.

Project implementation period: 2025.07.01 – 2025.10.31

Project budget: 100.000.000 Ft

Project aid intensity: 100%

Project location:
1094 Budapest, Liliom u. 43.
1094 Budapest, Tűzoltó u. 59.
7673 Kővágószőlős 524/1.

Project ID No: 2020-2.1.1-ED-2021-00190

Project title
Establishment of infrastructure for the basic research programme for the restoration of vision and for the basic research processes for the diagnosis and therapy of other central nervous system diseases.

Short summary of the project:
By implementing this application, BrainVisionCenter Nonprofit Kft. undertakes to establish the research infrastructure necessary to launch a research programme spanning almost 12 years, the part covered by this application, and the technical equipment necessary to implement the research plans. This technical equipment is a neurophotonic, acousto-optical technical development that goes beyond the current frontiers of science and technology with a multidisciplinary approach, and represents a valid alternative for the leaders of the scientific field. The research programme is expected to contribute to the acquisition of scientific degrees by a number of Hungarian researchers, to the retention of research excellence, to their professional fulfilment and/or to their return home, in such a way that Hungary’s scientific and technical advantage in the development of neurophotonics and acousto-optical systems will not only be maintained but also increased.

Project implementation period: 2021.12.28 – 2022.12.27

Project budget: 1.099.000.000 Ft

Project aid intensity: 100%

Project location:
1094 Budapest, Liliom u. 43.
1094 Budapest, Tűzoltó u. 59.
7673 Kővágószőlős 524/1.

Project ID No:
2020-2.1.1-ED-2022-00208

Project title:
Development of neurophotonic, molecular biological, neurochemical methods, brain interfaces and automated measurement systems for the development of therapies and diagnostics for vision restoration and other central nervous system diseases

Short summary of the project:
The project will develop a high-throughput automated robotic microscope for high throughput selection of AAV vectors for human retinal therapeutic procedures. The method uses 3D, fast, acousto-optical microscopy to measure the transfer function (input-output characteristics) of retinal samples, selecting the AAV vector constructs that most efficiently restore human retinal function. The project also aims to rapidly measure the brain function of marmosets in 3D to test different brain-computer interfaces and methods. This will include testing the 3D acousto-optical microscope itself as a possible version of the brain-computer interface. The work will also include the development of voltage-sensitive fluorescent dyes, photoactive materials for 3D in vivo measurements and fluorescent dyes for diagnostic purposes. With these solutions, the BrainVisionCenter’s research infrastructure can serve as a service centre providing access to state-of-the-art multiphoton techniques and modern neurophotonic methods for contracted researchers, companies, students, and doctoral and postdoctoral students, thus contributing to the development of the Hungarian high-tech biotechnology industry, the retention of a high-level scientific research base, and the repatriation of Hungarian researchers. The acousto-optical deflector development and manufacturing capacity created during the project will help Hungary, which was the world leader in this segment in the 1990s and still has an outstanding theoretical knowledge base, to regain its leading position.

Project implementation period: 2022.05.01 – 2025.06.30

Project budget: 6.200.000.000 Ft

Project aid intensity: 100%

Project location:
1094 Budapest, Liliom u. 43.
1094 Budapest, Tűzoltó u. 59.
7673 Kővágószőlős 524/1.