GFZnP-ED

Technology Overview

As the second most common transition metal in the body, the detection of Zn2+ is a necessity in many fields of applications. It has been related to neurodegenerative diseases, such as Alzheimer’s and Parkinson’s, or to important viral strains, such as the SARS-Cov-2. Therefore, we have developed three brand new fluorescent Zn2+ sensors, which have the capacity for spatial and temporal monitoring, which has not been the case with previous methods. These are sensors for one and two-photon microscopy, which are the two most available, sensitive and inexpensive methods for Zn2+ monitoring, with the aid of the high solubility, high fluorescens and optimal selectivity.

Features

  • Name: (Z)-5-((8-((2-(dimethylamino)ethyl)(methyl)amino)quinolin-2-yl)methylene)-2-(phenylamino)-3,5-dihydro-4H-imidazol-4-one
  • Molecular formula: C24H26N6O
  • MW: 414.50 Da
  • Standard packaging size: 250 µL, 5 mM

Benefits

    • Highest dynamic range (ΔF/F0 = 77) of our Zn2+ probes in one-photon setups
    • The excitation wavelength is 450 nm, while the emission is 509 nm.
    • The 2P-excitation wavelength is 900 nm

References

Two-photon fluorescent chemosensors based on the GFP-chromophore for the detection of Zn2+ in biological samples – From design to application
A. Csomos, E. Kovács, M. Madarász, F. Zs. Fedor, A. Fülöp, G. Katona, B. Rózsa, Z. Mucsi, Sensors and Actuators B: Chemical (2023)